yego.me
💡 Stop wasting time. Read Youtube instead of watch. Download Chrome Extension

Impact of mutations on translation into amino acids | High school biology | Khan Academy


5m read
·Nov 11, 2024

So let's start looking at a short sequence of DNA and the letters. I'm going to use these as the shorthands for the various nucleotide bases that make up a sequence of DNA.

So let's say that I have some thymine, thymine, cytosine, guanine, cytosine, thymine, adenine, thymine, thymine, and let's throw another thymine in there. So that would be our sequence of DNA.

What would be the corresponding sequence of RNA that it would be transcribed into? If you remember this from previous videos, pause this video and try to figure that out.

Well, the key thing to appreciate is if we're talking about base pairs in DNA, adenine pairs with thymine, cytosine pairs with guanine. But if we're talking about pairing into RNA, well then instead of thymine in the RNA, you would have uracil.

So the RNA here is, well, the thymine in the DNA would correspond to an adenine in the RNA: adenine, guanine, cytosine, guanine, adenine. And now since this is an RNA strand, instead of having a thymine right over here, this would be a uracil: adenine, adenine.

So this process that we just did, this is transcription, transcription, transcription from DNA to RNA. Now, the next step, if we're talking about the whole process of how does this information actually have an effect on the body, is we're going to go from the RNA and translate that into a protein.

The way we do that, we've seen this in previous videos, is every three of these bases—that's a codon—and it codes for a particular amino acid. Now, to figure out what amino acid it codes for, we look at an amino acid translation table and there are different types that you might see. This is the most typical type.

So the first base is A, second base A, third base is G. First base A, second base A, third base is G, and so that will code for the amino acid lysine. So we could write L-Y-S, short for lysine, here. And we could have also gotten that from a different type of translation table. For example, you might see a circular one that looks like that, but we would have gotten the same result. AAG, start at the center, AAG codes for lysine.

Then the next codon, and if you're getting as excited about this as I am, I encourage you to pause this video and try to keep translating this. The next codon is CGA, C-G-A, arginine. Arginine.

And then the next one is UAA. U-A-A. Well, here they have this little black circular dot. What does that mean? Well, that means stop codon, and sometimes they'll just write the word stop there. So this is stop. There is not an amino acid called stop; this actually signals, and this is happening at a ribosome, this is signaling for the translation process to stop.

This is the end of our amino acid chain, of our polypeptide chain, and so we will stop right over there. But now let's do some interesting things. Let's think about situations where there are mutations; where some of these bases, maybe something gets inserted, maybe something gets deleted, maybe something gets swapped out.

And so let's start with what's known as a point mutation. So let's say this C gets swapped out for an A. Well, if that happened, then on the RNA strand, all of a sudden, this would be a uracil. And if that is a uracil, this AAG would still be there coding for lysine, but this second codon is now different.

What would it now code for? Well, C U A. C-U-A, it'll now code for leucine instead of arginine. Leucine, L-E-U. This is fairly typical for a substitution mutation; it might change a particular amino acid. But sometimes it could be more significant.

For example, if this G was swapped out for an A, then this C on the RNA would then be a U, and then what would happen? Well, this first codon would still code for lysine, but the second one would be UGA. U-G-A. Now all of a sudden, it codes for a stop codon.

And so the actual translation process would stop, which could be a very, very big deal. If this DNA sequence, if the normal non-mutated polypeptide had to keep going on and on and on over here, just happened to have a stop codon next, but you can imagine if they had just, you know, another thousand codons before the end, but all of a sudden, you had a point mutation to stop early, that would significantly affect the protein that it is coding for.

Now, another type of mutation that typically has a fairly significant effect is a frameshift mutation. And that's where something gets inserted and/or deleted and shifts everything. So, for example, instead of the A being swapped in for the G, what if the A got inserted here? So then our sequence would look like this: T-T-C, and then we have an A, and then you have G-C-T, G-C-T, A-T-T-T.

So what just happened here? This was our original sequence, but the A got inserted here; it didn't replace the G, and so everything got shifted to the right. Now, what are we coding for? Well, when we transcribe to RNA, this will be AAG, UCG, AUA-A-A. And now this first codon still codes for lysine; we've seen that multiple times.

But what about this second codon? The second codon over here, UCG, U-C-G, that's serine. We got a different amino acid, and what's interesting is it's not just that one amino acid is changing; we're going to see that keeps happening.

So now we have UAA. U-A-A, here we have isoleucine. So iso right over here, which is different than what we had before. We now don't have a stop codon anymore, and we would keep going on and on, and so you can imagine a frameshift mutation where you either insert something or take it out so that the whole frame gets shifted.

Can have a dramatic impact on what it will transcribe and then translate for. Now lucky for us, even though mutations are always going on, there are many proofreading mechanisms in biological systems to make them less frequent than they otherwise would be, and people are still understanding how these proofreading mechanisms fully happen.

Another thing to appreciate is we often associate a mutation as being equal to a bad thing, and oftentimes it is a bad thing. What used to be a functional protein may no longer be a functional protein because the amino acids, the coding got stopped short or there was a frameshift mutation; it's just coding for completely different things.

So sometimes it could be very bad, and some diseases actually are caused by strange mutations like that that show up. Oftentimes, the mutation might not be a big deal; maybe something gets swapped out, maybe only one amino acid changes, and it doesn't really change the ability of the protein to do its job, in which case it doesn't matter.

But every now and then, a mutation can actually be a good thing. In fact, we need the mutation in order to have variation in a population, and variation is what natural selection and evolution run off. If you don't have variation, then you're not going to have different things that get selected in different environments, and you're not going to have that gradual change over time.

So big picture, hopefully you got a better appreciation for how transcription and then translation. Let me write that down. So that's transcription from DNA to RNA, and then this is translation, translation from RNA to protein.

We've got appreciation of how that happens. We've got appreciation of how to use these translation tables, but also how either a point mutation or frameshift mutation can eventually affect the protein that gets coded for.

More Articles

View All
What happened with my Property Manager…
What’s up you guys? It’s Graham here! So first of all, thank you for watching. Make sure to sit back, relax, subscribe, hit the like button, and let me give you some backstory on the situation: my experience hiring a property manager and whether or not a…
Nearly 100 Captive Orcas and Belugas at Risk of Drowning, Freezing to Death | National Geographic
This video from November 2018 shows a holding facility near the small Russian town of Nicosia, where government officials are investigating the capturing and exporting of wild beluga whales and orcas. This is footage of the same facility taken in January …
Geometric constructions: perpendicular line through a point off the line | Geometry | Khan Academy
What I have here is a line, and I have a point that is not on that line. My goal is to draw a new line that goes through this point and is perpendicular to my original line. How do I do that? Well, you might imagine that our compass will come in handy; i…
The common-ion effect | Equilibrium | AP Chemistry | Khan Academy
The presence of a common ion can affect a solubility equilibrium. For example, let’s say we have a saturated solution of lead(II) chloride. Lead(II) chloride is a white solid. So, here’s the white solid on the bottom of the beaker, and the solid is at equ…
The Nernst equation | Applications of thermodynamics | AP Chemistry | Khan Academy
We already know how to calculate cell potential when the reactants and products are in their standard states. However, what if that’s not the case? We can find cell potential when reactants and products are not in their standard states by using the Nernst…
THE 18-YEAR-OLD who sold $10-MILLION in Real Estate his FIRST YEAR (How He Did It)
That’s how I got my first open houses. They send an office-wide email, I was on my phone, I was found in a minute. Later, a few weeks later, I closed my first deal just under 3.2 million. Since then, I’ve closed six deals; the seventh will be closing in t…